Recombinant Human GNB3 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23096

Novus Biologicals, part of Bio-Techne
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NBP2-23096
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all GNB3 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-340 of Human GNB3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGEMEQL RQEAEQLKKQ IADARKACAD VTLAELVSGL EVVGRVQMRT RRTLRGHLAK IYAMHWATDS KLLVSASQDG KLIVWDSYTT NKVHAIPLRS SWVMTCAYAP SGNFVACGGL DNMCSIYNLK SREGNVKVSR ELSAHTGYLS CCRFLDDNNI VTSSGDTTCA LWDIETGQQK TVFVGHTGDC MSLAVSPDFN LFISGACDAS AKLWDVREGT CRQTFTGHES DINAICFFPN GEAICTGSDD ASCRLFDLRA DQELICFSHE SIICGITSVA FSLSGRLLFA GYDDFNCNVW DSMKSERVGI LSGHDNRVSC LGVTADGMAV ATGSWDSFLK IWN

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

39.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GNB3 His Protein

SDS-PAGE: Recombinant Human GNB3 His Protein [NBP2-23096]

SDS-PAGE: Recombinant Human GNB3 His Protein [NBP2-23096]

SDS-Page: GNB3 Protein [NBP2-23096]

Formulation, Preparation and Storage

NBP2-23096
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GNB3

GNB3 is important regulator of alpha subunit, as well as of certain signal transduction receptor and effector. A single-nucleotide polymorphism (C825T) in this protein is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Additional splice variants may exist for this gene, but they have not been fully described. Recombinant human GNB3 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

G protein, beta-3 subunit, GTP-binding regulatory protein beta-3 chain, guanine nucleotide binding protein (G protein), beta polypeptide 3, guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3, guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, hypertension associated protein, Transducin beta chain 3

Gene Symbol

GNB3

Additional GNB3 Products

Product Documents for Recombinant Human GNB3 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human GNB3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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