Recombinant Human GPR4 GST (N-Term) Protein

Name: Recombinant Human GPR4 GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00002828-P01

Novus Biologicals, part of Bio-Techne | Catalog # H00002828-P01

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Affinity Purification, Microarray, SDS-PAGE, Western Blot

View all GPR4 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-362 of Human GPR4

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MGNHTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVQQRNELGVYLMNLSIADLLYICTLPLWVDYFLHHDNWIHGPGSCKLFGFIFYTNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVSSVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMEGWVAWMNLYRVFVGFLFPWALMLLSYRGILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

67.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GPR4 GST (N-Term) Protein

SDS-PAGE: Recombinant Human GPR4 GST (N-Term) Protein [H00002828-P01]

SDS-Page: Recombinant Human GPR4 Protein [H00002828-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Formulation, Preparation and Storage

H00002828-P01

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: GPR4

GPR4 is a Lysophospholipid/Lysosphingolipid receptor. Recently, GPR4 has been shown to act as a proton-sensing receptor stimulating inositol phosphate formation (Ludwig et al. 2003). GPR4 has been reported in kidney, heart, and especially lung, where it is at least five-fold greater than in other tissues (Mahadevan et al., 1995). ESTs have been isolated from brain, colon, kidney, lung, and melanocyte/uterus/fetal heart libraries.

Long Name

G Protein-coupled Receptor 4

Alternate Names

GPR19

Gene Symbol

GPR4

Additional GPR4 Products

Product Documents for Recombinant Human GPR4 GST (N-Term) Protein

Product Datasheets

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COA

SDS

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Product Specific Notices for Recombinant Human GPR4 GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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