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Recombinant Human GPT2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18182

Novus Biologicals, part of Bio-Techne
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NBP3-18182-100ug
NBP3-18182-10ug
NBP3-18182-20ug
NBP3-18182-250ug
NBP3-18182-500ug
NBP3-18182-50ug

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

Product Specifications

Description

A recombinant protein with a His-Tag corresponding to amino acids 1-523 of Human GPT2

Source: E. coli

Accession Number: NP_597700

Amino Acid Sequence: MQRAAAL VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIEL ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP LYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQG AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV LQKVKDFHIN FLEKYA

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

60.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 100units/mg, and is defined as the amount of enzyme that cleaves 1 umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GPT2 His Protein

SDS-PAGE: Recombinant Human GPT2 His Protein [NBP3-18182]

SDS-PAGE: Recombinant Human GPT2 His Protein [NBP3-18182]

SDS-Page: Recombinant Human GPT2 His Protein [NBP3-18182] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP3-18182
Formulation 20mM Tris-HCl buffer (pH 7.5), 30% glycerol, 2mM DTT, 0.2M NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPT2

GPT (MIM 138200) and GPT2 (EC 2.6.1.2), also known as alanine transaminases, are pyridoxal enzymes that catalyze the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. By mediating the conversion of these 4 major intermediate metabolites, these transaminases have roles in gluconeogenesis and in amino acid metabolism.[supplied by OMIM]

Alternate Names

AAT2, alanine aminotransferase 2, ALT2EC 2.6.1.2, Glutamate pyruvate transaminase 2, glutamic pyruvate transaminase (alanine aminotransferase) 2, Glutamic--alanine transaminase 2, glutamic-pyruvate transaminase 2, Glutamic--pyruvic transaminase 2, GPT 2

Gene Symbol

GPT2

Additional GPT2 Products

Product Documents for Recombinant Human GPT2 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human GPT2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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