Recombinant GST Epitope Tag Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18537

Novus Biologicals, part of Bio-Techne
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NBC1-18537
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all GST Epitope Tag Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-218 of Schistosoma japonicum E.coli (Bioactivity Validated) GST Epitope Tag

Source: E.coli

Amino Acid Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 20 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Application Notes

Use in functional reported in scientific literature (PMID 26945068).

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant GST Epitope Tag Protein

SDS-PAGE: Recombinant GST Epitope Tag Protein [NBC1-18537]

SDS-PAGE: Recombinant GST Epitope Tag Protein [NBC1-18537]

SDS-Page: Recombinant Parasite GST Epitope Tag Protein [NBC1-18537] - GST Epitope Tag, 26kDa (224aa) with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-18537
Formulation PBS (pH 7.4)
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GST Epitope Tag

Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST Epitope Tag may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E.coli source. Recombinant GST Epitope Tag was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

EC 2.5.1.18, glutathione S-alkyltransferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase M1, glutathione S-transferase mu 1, GST class-mu 1, GST HB subunit 4, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, HB subunit 4, MGC26563, MU, MU-1, S-(hydroxyalkyl)glutathione lyase

Gene Symbol

GSTM1

Additional GST Epitope Tag Products

Product Documents for Recombinant GST Epitope Tag Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant GST Epitope Tag Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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