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Histone H1.5 Recombinant Protein Antigen

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-17162PEP

Novus Biologicals, part of Bio-Techne
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NBP3-17162PEP

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Antibody Competition

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human Histone H1.5

Source: E. coli

Amino Acid Sequence: ETATPAPVEKSPAKKKATKKAAG

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10-100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP3-17162.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation and Storage

NBP3-17162PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H1.5

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Histone H1.5 is a 207 amino acid long, 22 kDA protein encoded by the gene HIST1H1B (found on chromosome 6p22-p21.3) that lacks polyA tails and instead obtain palindromic termination elements. H1 histones are critical for the condensation of nucleosome chains into higher order structures. HIST1H1B has been investigated regarding breast cancer, measles, ataxia, t-cell leukemia, arthritis, alzheimer's diseases, lupus, and prostate cancer. It is active in apoptosis, histone modification, PKA signaling, and cell cycle chromosome condensation in prometaphase as it interacts with genes H3F3A, H3F3B, SIRT1, CDK2, and L3MNTL1.

Alternate Names

H1, H1 histone family, member 5, H1.5, H1F5H1B, histone 1, H1b, histone cluster 1, H1b, histone H1.5, Histone H1a, MGC126630, MGC126632

Gene Symbol

HIST1H1B

Additional Histone H1.5 Products

Product Documents for Histone H1.5 Recombinant Protein Antigen

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histone H1.5 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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