Recombinant Human Histone H2AX His Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42464
Key Product Details
Source
E. coli
Tag
His
Conjugate
Unconjugated
Applications
SDS-PAGE, Western Blot
Product Specifications
Description
Recombinant H2AX protein corresponding to amino acids 2 - 143.
Source: E. coli
(Accession #: P16104)
Purity
>90%, by SDS-PAGE
Predicted Molecular Mass
17.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Application Notes
This product is useful as a positive control for both Western Blot and SDS-Page. This protein has been tested using NB100-638 for detection on a Western Blot.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human Histone H2AX His Protein
![Western Blot: Recombinant Human Histone H2AX His Protein [NBP1-42464]](https://resources.bio-techne.com/images/products/H2AX-Protein-Western-Blot-NBP1-42464-img0002.jpg "Western Blot: Recombinant Human Histone H2AX His Protein [NBP1-42464]")
Western Blot: Recombinant Human Histone H2AX His Protein [NBP1-42464]
Western Blot: H2AX Protein [NBP1-42464] - The anti-H2AX antibody NB100-638 was used to detect this protein. 200ng of protein was loaded onto the gel and NB100-638 was used to detect the protein.![SDS-PAGE: Recombinant Human Histone H2AX His Protein [NBP1-42464]](https://resources.bio-techne.com/images/products/H2AX-Protein-Immunoblotting-NBP1-42464-img0001.jpg "SDS-PAGE: Recombinant Human Histone H2AX His Protein [NBP1-42464]")
SDS-PAGE: Recombinant Human Histone H2AX His Protein [NBP1-42464]
SDS-Page: H2AX Protein [NBP1-42464] - This H2AX protein was tested on a 4-20% SDS-PAGE gel with 5ug of protein loaded on the gel. The purity of the protein is >95%.Formulation, Preparation and Storage
NBP1-42464
| Formulation | 25 mM Tris pH 8.0, 250 mM NaCl, 8 M Urea, 1 mM DTT |
| Preservative | N/A |
| Concentration | 0.5 mg/ml |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -80C. Avoid freeze-thaw cycles. |
Background: Histone H2AX
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Recombinant Human Histone H2AX His Protein
Product Specific Notices for Recombinant Human Histone H2AX His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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