Horse IgG, Fab2 Native Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96781
![SDS-PAGE: Horse IgG, Fab2 Native Protein [NBP1-96781]](https://resources.bio-techne.com/images/products/Horse-IgG--Fab2-Native-Protein-SDS-Page-NBP1-96781-img0001.jpg "SDS-PAGE: Horse IgG, Fab2 Native Protein [NBP1-96781]")
Key Product Details
Conjugate
Unconjugated
Applications
SDS-PAGE, Western Blot
Product Specifications
Description
Native Equine Horse IgG, Fab2
Source: Normal serum
Store vial at 4C upon receipt. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Source: Normal serum
Store vial at 4C upon receipt. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Purity
Multi-step
Applications
Western Blot (1:100-1:2000)
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Protein / Peptide Type
Native Protein
Scientific Data Images for Horse IgG, Fab2 Native Protein
SDS-PAGE: Horse IgG, Fab2 Native Protein [NBP1-96781]
SDS-Page: Horse IgG, Fab2 Native Protein [NBP1-96781] - SDS-PAGE of Horse F(ab')2 Fragment. Lane 1: 1x Reduced, Horse F(ab')2 Fragment. Lane 2: Molecular Weight Ladder. 4-20% SDS-PAGE; Coomassie Stained.Formulation, Preparation and Storage
NBP1-96781
| Preparation Method | This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse IgG, anti-Horse IgG F(ab')2 and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(c) or anti-Pepsin. |
| Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: Horse IgG, Fab2
Additional Horse IgG, Fab2 Products
Product Documents for Horse IgG, Fab2 Native Protein
Product Specific Notices for Horse IgG, Fab2 Native Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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