Human Plasminogen Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1939-SE
Key Product Details
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Plasminogen protein
The human plasma used for the isolation of this product was certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.
The human plasma used for the isolation of this product was certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
EPLDDYNVTQ (major) and LDDYNVTQGA (minor)
SDS-PAGE
100-106 kDa doublet, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, SUC-AFK-AMC.
The specific activity, measured under the described conditions, is >500 pmol/min/µg.
The specific activity, measured under the described conditions, is >500 pmol/min/µg.
Formulation, Preparation and Storage
1939-SE
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 1 mg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 8.0. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Plasminogen
Plasminogen (PLG) is the precursor of plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion (1, 2). Synthesized in the kidney, PLG is found in plasma and many extracellular fluids. Activated by u- or t-plasminogen activator, the single-chain PLG (amino acid residues 20‑810) is converted to plasmin, which consists of disulfide bond-linked heavy chain A (residues 20-580) and light chain B (residues 581‑810). Heavy chain A contains 5 kringle domains and light chain B corresponds to the serine protease domain. A fragment consisting of the first 4 kringle domains has been named as angiostatin, a novel angiogenesis inhibitor (3, 4).
References
- Petersen, T.E. et al. (1990) J. Biol. Chem. 265:6104.
- Forsgren, M. et al. (1987) FEBS Lett. 213:254.
- O’Reilly, M.S. et al. (1994) Cell 79:315.
- Sim, B.K. et al. (1997) Cancer Res. 57:1329.
Alternate Names
Plg
Gene Symbol
PLG
Additional Plasminogen Products
Product Documents for Human Plasminogen Protein, CF
Product Specific Notices for Human Plasminogen Protein, CF
For research use only
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