Recombinant Human HUS1 His Protein

Name: Recombinant Human HUS1 His Protein
Brand: Novus Biologicals
SKU: NBP1-50899

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50899

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all HUS1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-280 of Human HUS1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKFRAKIVDG ACLNHFTRIS NMIAKLAKTC TLRISPDKLN FILCDKLANG GVSMWCELEQ ENFFNEFQME GVSAENNEIY LELTSENLSR ALKTAQNARA LKIKLTNKHF PCLTVSVELL SMSSSSRIVT HDIPIKVIPR KLWKDLQEPV VPDPDVSIYL PVLKTMKSVV EKMKNISNHL VIEANLDGEL NLKIETELVC VTTHFKDLGN PPLASESTHE DRNVEHMAEV HIDIRKLLQF LAGQQVNPTK ALCNIVNNKM VHFDLLHEDV SLQYFIPALS

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

33.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human HUS1 His Protein

SDS-PAGE: Recombinant Human HUS1 His Protein [NBP1-50899]

SDS-Page: Recombinant Human HUS1 Protein [NBP1-50899] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-50899

Formulation	20 mM Tris-HCl buffer (pH 8.0), 100 mM NaCl, 40% Glycerol
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HUS1

HUS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex. This protein associates with Rad9 and Rad1 to form the 9-1-1(RAD9-RAD1-HUS1) complex, which localizes to DNA lesions and promotes DNA damage signaling and repair or apoptosis, cell cycle arrest. The trimeric complex is structurally similar to the proliferating cell nuclear antigen (PCNA) sliding clamp and interacts with Rad17 as a clamp-clamp loader pair during the DNA damage response. Recombinant human HUS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

checkpoint protein HUS1, hHUS1, HUS1 (S. pombe) checkpoint homolog, HUS1 checkpoint homolog (S. pombe), hus1+-like protein

Gene Symbol

HUS1

Additional HUS1 Products

Product Documents for Recombinant Human HUS1 His Protein

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COA

Product Specific Notices for Recombinant Human HUS1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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