Recombinant Human IGF-II/IGF2 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59722
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 67 amino acids corresponding to IGF-II/IGF2 Source: E. coli
Uniprot ID: E3UN46
Amino Acid Sequence: AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of IGF-II/IGF2 as determined by LAL method.
Predicted Molecular Mass
7.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
IGF-II/IGF2 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human IGF-II/IGF2 Protein
![SDS-PAGE: Recombinant Human IGF-II/IGF2 Protein [NBP2-59722]](https://resources.bio-techne.com/images/products/Recombinant-Human-IGF-II-Protein-SDS-Page-NBP2-59722-img0002.jpg "SDS-PAGE: Recombinant Human IGF-II/IGF2 Protein [NBP2-59722]")
SDS-PAGE: Recombinant Human IGF-II/IGF2 Protein [NBP2-59722]
SDS-Page: Recombinant Human IGF-II Protein [NBP2-59722] - Recombinant IGF-II Protein [NBP2-59722] - 98 % by SDS-PAGE and HPLC analyses.Formulation, Preparation and Storage
NBP2-59722
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 0.02 % Tween-20. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: IGF-II/IGF2
Long Name
Insulin-like Growth Factor II
Alternate Names
C11orf43, GRDF, IGF-2, IGF2, IGFII, MSA, PEG2, PP9974
Gene Symbol
IGF2
Additional IGF-II/IGF2 Products
Product Documents for Recombinant Human IGF-II/IGF2 Protein
Product Specific Notices for Recombinant Human IGF-II/IGF2 Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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