Recombinant Mouse IL-19 Protein

Name: Recombinant Mouse IL-19 Protein
Brand: Novus Biologicals
SKU: NBP2-61330

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61330

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, Western Blot

View all IL-19 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-19 Source: E. coli

Uniprot ID: Q8CJ70

Amino Acid Sequence: LRRCLISVDM RLIEKSFHEI KRAMQTKDTF KNVTILSLEN LRSIKPGDVC CMTNNLLTFY RDRVFQDHQE RSLEVLRRIS SIANSFLCVQ KSLERCQVHR QCNCSQEATN ATRIIHDNYN QLEVSSAALK SLGELNILLA WIDRNHLETP AA

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of IL-19 as determined by LAL method.

Predicted Molecular Mass

17.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-19 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Ra and human IL-20Rb co-transfected murine BaF3 pro-B cells is less than 0.8 ng/ml, corresponding to a specific activity of > 1.25 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse IL-19 Protein

SDS-PAGE: Recombinant Mouse IL-19 Protein [NBP2-61330]

SDS-Page: Recombinant Mouse IL-19 Protein [NBP2-61330]

Formulation, Preparation and Storage

NBP2-61330

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 3% Trehalose.
Preservative	Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-19

IL19 is encoded by this gene is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described. [provided by RefSeq]

Long Name

Interleukin 19

Alternate Names

IL19

Gene Symbol