Recombinant Human IL-20 Protein

Name: Recombinant Human IL-20 Protein
Brand: Novus Biologicals
SKU: NBP2-35056

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35056

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9NYY1

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-20 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 153 amino acids corresponding to IL-20 Source: E. coli

Uniprot ID: Q9NYY1

Amino Acid Sequence: MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-20 as determined by LAL method.

Predicted Molecular Mass

17.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-20 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20R alpha and human IL-20R beta co-transfected murine BaF3 pro-B cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-20 Protein

SDS-PAGE: Recombinant Human IL-20 Protein [NBP2-35056]

SDS-Page: Human IL-20 Protein [NBP2-35056]

Formulation, Preparation and Storage

NBP2-35056

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.2.
Preservative	Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-20

Human Interleukin-20 (IL-20) is encoded by the IL20 gene located on the chromosome 1 and belongs to the IL-10 family that including IL-10, IL-19, IL-22, IL-24, and IL-26. It is secreted by activated keratinocytes and monocytes, and signals through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. IL-20 has functions of regulating proliferation and differentiation of keratinocytes during inflammation, and causing cell expansion of multipotential hematopoietic progenitor cells.

Long Name

Interleukin 20

Alternate Names

IL20

Gene Symbol