Recombinant Human IL-22 Protein

Name: Recombinant Human IL-22 Protein
Brand: Novus Biologicals
SKU: NBP2-34861

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34861

Submit a review

Product Datasheet / COA

Catalog #

Availability

Size / Price

Qty

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9GZX6

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all IL-22 Proteins and Enzymes »

Product Specifications

Description

A non-disulfide-linked homodimeric protein containing two 147 amino acid polypeptide chains corresponding to IL-22 Source: E. coli

Uniprot ID: Q9GZX6

Amino Acid Sequence: MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-22 as determined by LAL method.

Predicted Molecular Mass

33.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL22 Protein is fully biologically active when compared to standard. The ED50 as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.3 ng/ml, corresponding to a specific activity of > 3.3 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-22 Protein

SDS-PAGE: Recombinant Human IL-22 Protein [NBP2-34861]

SDS-Page: Recombinant Human IL-22 Protein [NBP2-34861]

Formulation, Preparation and Storage

NBP2-34861

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 5.0.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-22

IL-22 is belonging to IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes and dendritic cells, IL-10 contributes to the inflammatory response in vivo. IL-22 signals through CRF2-4 and IL-22. It along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems.

Long Name

Interleukin 22

Alternate Names

IL-TIF, IL22

Gene Symbol

IL22

UniProt