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Recombinant Human IL-36 gamma/IL-1F9 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34891

Novus Biologicals, part of Bio-Techne
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NBP2-34891-100ug
NBP2-34891-10ug
NBP2-34891-1mg
NBP2-34891-500ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-36 gamma/IL-1F9 Source: E. coli

Uniprot ID: Q9NZH8

Amino Acid Sequence: SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of IL-36 gamma/IL-1F9 as determined by LAL method.

Predicted Molecular Mass

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

IL-36 gamma/IL-1F9 protein is fully biologically active when compared to standard. The ED50 as determined by its ability to induce IL-8 secretion by human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of > 1 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL-36 gamma/IL-1F9 Protein

SDS-PAGE: Recombinant Human IL-36 gamma/IL-1F9 Protein [NBP2-34891]

SDS-PAGE: Recombinant Human IL-36 gamma/IL-1F9 Protein [NBP2-34891]

SDS-Page: Recombinant Human IL-36 gamma/IL-1F9 Protein [NBP2-34891]

Formulation, Preparation and Storage

NBP2-34891
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: IL-36 gamma/IL-1F9

Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36beta, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36gamma is secreted when transfected into 293-T cells and it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). Furthermore, IL-36gamma also can function as an agonist of NF-kappa B activation through the orphan IL-1- receptor-related protein 2. Human IL-36gamma (152a.a) shares 58 %, 59 %, 68 % and 69 % a.a. sequence identity with mouse, rat, bovine and equine IL-36gamma, respectively, and 23 - 57% a.a. sequence identity with other family members.

Long Name

Interleukin 36 gamma/Interleukin 1 Family 9

Alternate Names

IL-1 H1, IL-1rp2, IL1F9, IL36 gamma, IL36G

Gene Symbol

IL36G

UniProt

Additional IL-36 gamma/IL-1F9 Products

Product Documents for Recombinant Human IL-36 gamma/IL-1F9 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human IL-36 gamma/IL-1F9 Protein

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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