Recombinant Human IL12B His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99042

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

Baculovirus

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all IL12B Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 23-328 of Human IL12B

Source:Baculovirus

Amino Acid Sequence: ADPIWELKKD VYVVELDWYP DAPGEMVVLT CDTPEEDGIT WTLDQSSEVL GSGKTLTIQV KEFGDAGQYT CHKGGEVLSH SLLLLHKKED GIWSTDILKD QKEPKNKTFL RCEAKNYSGR FTCWWLTTIS TDLTFSVKSS RGSSDPQGVT CGAATLSAER VRGDNKEYEY SVECQEDSAC PAAEESLPIE VMVDAVHKLK YENYTSSFFI RDIIKPDPPK NLQLKPLKNS RQVEVSWEYP DTWSTPHSYF SLTFCVQVQG KSKREKKDRV FTDKTSATVI CRKNASISVR AQDRYYSSSW SEWASVPCSH HHHHH

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

35.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human IL12B His Protein

SDS-PAGE: Recombinant Human IL12B His Protein [NBP1-99042]

SDS-PAGE: Recombinant Human IL12B His Protein [NBP1-99042]

SDS-Page: Recombinant Human IL12B Protein [NBP1-99042]

Formulation, Preparation and Storage

NBP1-99042
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 20% glycerol, 100 mM NaCl, 0.1 mM PMSF
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL12B

IL12P40 (interleukin 12 subunit beta), also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40, belongs to the type I cytokine receptor family. This protein is a cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. Recombinant human IL12P40 protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.

Alternate Names

CLMF, CLMF p40, CLMF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12BNK cell stimulatory factor chain 2, interleukin 12, p40, interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40), interleukin-12 beta chain, interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD subunit, NKSF, NKSF2IL12, subunit p40

Gene Symbol

IL12B

Additional IL12B Products

Product Documents for Recombinant Human IL12B His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL12B His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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