Recombinant E. coli Inorganic Pyrophosphatase/PPA1 His Protein

Name: Recombinant E. coli Inorganic Pyrophosphatase/PPA1 His Protein
Brand: Novus Biologicals
SKU: NBP1-72378

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72378

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Inorganic Pyrophosphatase/PPA1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-176 of E.coli Inorganic Pyrophosphatase/PPA1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE AAKAEIVASF ERAKNK

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

21.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli Inorganic Pyrophosphatase/PPA1 His Protein

SDS-PAGE: Recombinant E. coli Inorganic Pyrophosphatase/PPA1 His Protein [NBP1-72378]

SDS-Page: Recombinant E. coli Inorganic Pyrophosphatase/PPA1 Protein [NBP1-72378] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72378

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 50 mM NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Inorganic Pyrophosphatase/PPA1

Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations. Recombinant E.coli ppa protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

IOPPP, IPP1, PP1, PPase, SID6-8061

Gene Symbol

PPA1

Additional Inorganic Pyrophosphatase/PPA1 Products

Product Documents for Recombinant E. coli Inorganic Pyrophosphatase/PPA1 His Protein

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COA

Product Specific Notices for Recombinant E. coli Inorganic Pyrophosphatase/PPA1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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