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Recombinant Human ISCU His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50926

Novus Biologicals, part of Bio-Techne
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NBP1-50926-0.01mg
NBP1-50926-0.05mg

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 35-167 of Human ISCU

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MYHKKVVDHY ENPRNVGSLD KTSKNVGTGL VGAPACGDVM KLQIQVDEKG KIVDARFKTF GCGSAIASSS LATEWVKGKT VEEALTIKNT DIAKELCLPP VKLHCSMLAE DAIKAALADY KLKQEPKKGE AEKK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

16.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ISCU His Protein

SDS-PAGE: Recombinant Human ISCU His Protein [NBP1-50926]

SDS-PAGE: Recombinant Human ISCU His Protein [NBP1-50926]

SDS-Page: Recombinant Human ISCU Protein [NBP1-50926] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-50926
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 2 mM DTT, 100 mM NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ISCU

Iron-sulfur cluster assembly enzyme, also known as ISCU, is a member of the nifU family. Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. It is interact with ISCS, a cysteine desulfurase, to sequester inorganic sulfur for Fe-S cluster assembly. ISCU-ISCS protein complex localizes in both mitochondria and cytosol, implying that Fe-S cluster assembly takes place in multiple subcellular compartments in mammalian cells. Recombinant human ISCU protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

2310020H20Rik, HML, hnifU, iron-sulfur cluster assembly enzyme ISCU, mitochondrial, iron-sulfur cluster scaffold homolog (E. coli), IscU, IscU iron-sulfur cluster scaffold homolog, IscU iron-sulfur cluster scaffold homolog (E. coli), ISU2, MGC74517, NIFU, NifU-like N-terminal domain containing, NifU-like N-terminal domain-containing protein, NifU-like protein, NIFUN

Gene Symbol

ISCU

Additional ISCU Products

Product Documents for Recombinant Human ISCU His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human ISCU His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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