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Recombinant Human Josephin1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23156

Novus Biologicals, part of Bio-Techne
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NBP2-23156

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-202 of Human Josephin1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSCVPWK GDKAKSESLE LPQAAPPQIY HEKQRRELCA LHALNNVFQD SNAFTRDTLQ EIFQRLSPNT MVTPHKKSML GNGNYDVNVI MAALQTKGYE AVWWDKRRDV GVIALTNVMG FIMNLPSSLC WGPLKLPLKR QHWICVREVG GAYYNLDSKL KMPEWIGGES ELRKFLKHHL RGKNCELLLV VPEEVEAHQS WRTDV

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

25.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Josephin1 His Protein

SDS-PAGE: Recombinant Human Josephin1 His Protein [NBP2-23156]

SDS-PAGE: Recombinant Human Josephin1 His Protein [NBP2-23156]

SDS-Page: Josephin1 Protein [NBP2-23156]

Formulation, Preparation and Storage

NBP2-23156
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 40% glycerol, 5mM DTT, 2 mM EDTA
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Josephin-1/JOSD1

JOSD1, also as known as Josephin-1, has low protease activity towards poly-ubiquitin chains (in vitro). JOSD1 is act as a deubiquitinating enzyme. Recently identified ubiquitin-binding sites in the Josephin domain contribute to ubiquitin chain binding and cleavage. Recombinant human JOSD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Josephin Domain Containing 1

Alternate Names

EC 3.4.19.12, JOSD1, JSPH1

Gene Symbol

JOSD1

Additional Josephin-1/JOSD1 Products

Product Documents for Recombinant Human Josephin1 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Josephin1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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