Recombinant Mouse LIGHT/TNFSF14 Protein

Name: Recombinant Mouse LIGHT/TNFSF14 Protein
Brand: Novus Biologicals
SKU: NBP2-61332

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61332

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, Western Blot

View all LIGHT/TNFSF14 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 168 amino acids corresponding to LIGHT/TNFSF14 Source: E. coli

Amino Acid Sequence: DGGKGSWEKL IQDQRSHQAN PAAHLTGANA SLIGIGGPLL WETRLGLAFL RGLTYHDGAL VTMEPGYYYV YSKVQLSGVG CPQGLANGLP ITHGLYKRTS RYPKELELLV SRRSPCGRAN SSRVWWDSSF LGGVVHLEAG EEVVVRVPGN RLVRPRDGTR SYFGAFMV

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of LIGHT/TNFSF14 as determined by LAL method.

Predicted Molecular Mass

18.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

LIGHT/TNFSF14 protein is fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using human HT-29 cells is less than 2 ug/ml, corresponding to a specific activity of > 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and rHuIFN-g.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse LIGHT/TNFSF14 Protein

SDS-PAGE: Recombinant Mouse LIGHT/TNFSF14 Protein [NBP2-61332]

SDS-Page: Recombinant Mouse LIGHT/TNFSF14 Protein [NBP2-61332]

Formulation, Preparation and Storage

NBP2-61332

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in 100 mM HAC to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: LIGHT/TNFSF14

TNFSF14 is encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Long Name

TNF Ligand Superfamily Member 14

Alternate Names

CD258, TNFSF14

Gene Symbol

TNFSF14

Additional LIGHT/TNFSF14 Products

Product Documents for Recombinant Mouse LIGHT/TNFSF14 Protein

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COA

Product Specific Notices for Recombinant Mouse LIGHT/TNFSF14 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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