May I ask whether NBP1-30275 protein is functionally active?

Name: Recombinant Human LIN-28A His Protein
Brand: Novus Biologicals
SKU: NBP1-30275

This is a recombinant protein (His-tagged) and we have not tested its functional activity in our lab yet.

Recombinant Human LIN-28A His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30275

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all LIN-28A Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 42-209 of Human LIN-28A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH RSMGICKWFN VRMGFGFLSM TARAGVALDP PVDVFVHQSK LHMEGFRSLK EGEAVEFTFK KSAKGLESIR VTGPGGVFCI GSERRPKGKS MQKRRSKGDR CYNCGGLDHH AKECKLPPQP KKCHFCQSIS HMVASCPLKA QQGPSAQGKP TYFREEEEEI HSPTLLPEAQ N

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human LIN-28A His Protein

SDS-PAGE: Recombinant Human LIN-28A His Protein [NBP1-30275]

SDS-Page: Lin28 Protein [NBP1-30275] - Lin28, 21.1 kDa (191aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBP1-30275

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.1 M NaCl, 0.1 mM PMSF
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIN-28A

Lin-28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. It is marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. It has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Recombinant Lin28 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

RNA-binding Protein LIN-28

Alternate Names

CSDD1, LIN28, LIN28A, Tex17, ZCCHC1

Gene Symbol

LIN28A

Additional LIN-28A Products

Product Documents for Recombinant Human LIN-28A His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human LIN-28A His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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