Recombinant Human LMX1b His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-51742

Novus Biologicals, part of Bio-Techne
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NBP2-51742-0.1mg
NBP2-51742-0.5mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all LMX1b Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-395 of Human LMX1b

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDIATGP ESLERCFPRG QTDCAKMLDG IKMEEHALRP GPATLGVLLG SDCPHPAVCE GCQRPISDRF LMRVNESSWH EECLQCAACQ QALTTSCYFR DRKLYCKQDY QQLFAAKCSG CMEKIAPTEF VMRALECVYH LGCFCCCVCE RQLRKGDEFV LKEGQLLCKG DYEKEKDLLS SVSPDESDSV KSEDEDGDMK PAKGQGSQSK GSGDDGKDPR RPKRPRTILT TQQRRAFKAS FEVSSKPCRK VRETLAAETG LSVRVVQVWF QNQRAKMKKL ARRHQQQQEQ QNSQRLGQEV LSSRMEGMMA SYTPLAPPQQ QIVAMEQSPY GSSDPFQQGL TPPQMPGNDS IFHDIDSDTS LTSLSDCFLG SSDVGSLQAR VGNPIDRLYS MQSSYFAS

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

46.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human LMX1b His Protein

SDS-PAGE: Recombinant Human LMX1b His Protein [NBP2-51742]

SDS-PAGE: Recombinant Human LMX1b His Protein [NBP2-51742]

SDS-Page: LMX1b Recombinant Protein [NBP2-51742] - 15% SDS Page (3 ug)

Formulation, Preparation and Storage

NBP2-51742
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LMX1b

LMX1B is a gene that codes for a protein with three isoforms, measuring 402, 395, and 406 amino acids in length with weights of approximately 45, 44, and 45 kDa respectively. LMX1B is necessary for the specification of dorsal limb fate. Current studies are being done on several diseases and disorders related to this gene including Meier-Gorlin syndrome, steroid-resistant nephrotic syndrome, sex reversal, attention deficit hyperactivity disorder, cleft lip/palate, familial Mediterranean fever, genitopatellar syndrome, major depressive disorder, nail dysplasia, short stature, heart block, and neuronitis. LMX1B has also been shown to have interactions with LDB1, TCF3, SSBP3, ALX4, and LDB2 in pathways such as the SIDS susceptibility pathway.

Alternate Names

LIM homeobox transcription factor 1, beta, LIM homeobox transcription factor 1-beta, LIM/homeobox protein 1.2, LIM/homeobox protein LMX1B, LMX1.2, LMX-1.2, MGC138325, MGC142051, NPS1

Gene Symbol

LMX1B

Additional LMX1b Products

Product Documents for Recombinant Human LMX1b His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human LMX1b His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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