Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

Name: Recombinant Human Lymphotoxin-alpha/TNF-beta Protein
Brand: Novus Biologicals
SKU: NBP2-61307

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61307

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, Western Blot

View all Lymphotoxin-alpha/TNF-beta Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 171 amino acids corresponding to Lymphotoxin-alpha/TNF-beta Source: E. coli

Amino Acid Sequence: LPGVGLTPSA AQTARQHPKM HLAHSTLKPA AHLIGDPSKQ NSLLWRANTD RAFLQDGFSL SNNSLLVPTS GIYFVYSQVV FSGKAYSPKA TSSPLYLAHE VQLFSSQYPF HVPLLSSQKM VYPGLQEPWL HSMYHGAAFQ LTQGDQLSTH TDGIPHLVLS PSTVFFGAFA L

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Lymphotoxin-alpha/TNF-beta as determined by LAL method.

Predicted Molecular Mass

18.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Lymphotoxin-alpha/TNF-beta protein is fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 5 pg/ml, corresponding to a specific activity of > 2.0 x 10^8 IU/mg in the presence of actinomycin D.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

SDS-PAGE: Recombinant Human Lymphotoxin-alpha/TNF-beta Protein [NBP2-61307]

SDS-Page: Recombinant Human Lymphotoxin-alpha/TNF-beta Protein [NBP2-61307]

Formulation, Preparation and Storage

NBP2-61307

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4, with 0.02 % Tween-20.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Lymphotoxin-alpha/TNF-beta

TNF-beta (tumor necrosis factor beta, lymphotoxin alpha, TNFSF1, TNF ligand superfamily member 1) a member of the TNF ligand superfamily and a potent lymphoid factor, is mainly produced by activated T and B lymphocytes and leukocytes. TNF-beta is also produced by fibroblasts, astrocytes, myeloma cells, endothelial cells and epithelial cells. TNF-beta mediates inflammatory and immune responses, has cytotoxic activity on tumor cells and other target cells and shares many of the same bioactivities as TNF alpha, including regulation of cell proliferation, differentiation and apoptosis. TNF-beta is not species-specific and human TNF-beta is active on murine cells with a slightly reduced specific activity.

Long Name

Tumor Necrosis Factor beta

Alternate Names

LTA, Lymphotoxin alpha, Lymphotoxinalpha, TNF-beta, tnfb, TNFSF1, TNFSF1B

Gene Symbol

LTA

Additional Lymphotoxin-alpha/TNF-beta Products

Product Documents for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

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COA

Product Specific Notices for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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