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Recombinant Human MafF His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22941

Novus Biologicals, part of Bio-Techne
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NBP2-22941

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-164 of Human MafF

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSVDPLS SKALKIKREL SENTPHLSDE ALMGLSVREL NRHLRGLSAE EVTRLKQRRR TLKNRGYAAS CRVKRVCQKE ELQKQKSELE REVDKLAREN AAMRLELDAL RGKCEALQGF ARSVAAARGP ATLVAPASVI TIVKSTPGSG SGPAHGPDPA HGPASCS

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

20.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MafF His Protein

SDS-PAGE: Recombinant Human MafF His Protein [NBP2-22941]

SDS-PAGE: Recombinant Human MafF His Protein [NBP2-22941]

SDS-Page: MAFF Protein [NBP2-22941]

Formulation, Preparation and Storage

NBP2-22941
Formulation 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MafF

MAFF is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. This protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. Recombinant human MAFF protein, fused to His-tag at N-terminus, was expressed in E.coli

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog F

Alternate Names

hMafF, transcription factor MafF, U-MAF, v-maf avian musculoaponeurotic fibrosarcoma oncogene family protein F, v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein F, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)

Gene Symbol

MAFF

Additional MafF Products

Product Documents for Recombinant Human MafF His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human MafF His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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