Recombinant Human MANF Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34951
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing158 amino acids corresponding to MANF Source: E. coli
Uniprot ID: P55145
Amino Acid Sequence: LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of MANF as determined by LAL method.
Predicted Molecular Mass
18.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 ug/ml, corresponding to a specific activity of > 50 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human MANF Protein
![SDS-PAGE: Recombinant Human MANF Protein [NBP2-34951]](https://resources.bio-techne.com/images/products/Human-MANF-Protein-SDS-Page-NBP2-34951-img0001.jpg "SDS-PAGE: Recombinant Human MANF Protein [NBP2-34951]")
SDS-PAGE: Recombinant Human MANF Protein [NBP2-34951]
SDS-Page: Human MANF Protein [NBP2-34951]Formulation, Preparation and Storage
NBP2-34951
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: MANF
Long Name
Mesencephalic Astrocyte-derived Neurotrphic Factor
Alternate Names
ARMET, ARP
Gene Symbol
MANF
UniProt
Additional MANF Products
Product Documents for Recombinant Human MANF Protein
Product Specific Notices for Recombinant Human MANF Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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