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Recombinant Mouse MDH2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61450

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-61450-100ug
NBP2-61450-20ug

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-terminalHis-tag and corresponding to the amino acids 25-338 of Mouse MDH2.

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET RANVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMVCIIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL ATLTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVQSKET ECTYFSTPLL LGKKGLEKNL GIGKITPFEE KMIAEAIPEL KASIKKGEDF VKNMK

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

35.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 800 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse MDH2 His Protein

SDS-PAGE: Recombinant Mouse MDH2 His Protein [NBP2-61450]

SDS-PAGE: Recombinant Mouse MDH2 His Protein [NBP2-61450]

SDS-Page: Recombinant Mouse MDH2 Protein [NBP2-61450] - 15% SDS-Page (3ug)

Formulation, Preparation and Storage

NBP2-61450
Formulation PBS (pH 7.4), 20% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDH2

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. [provided by RefSeq]

Long Name

Malate dehydrogenase, mitochondrial

Alternate Names

EC 1.1.1.37

Gene Symbol

MDH2

Additional MDH2 Products

Product Documents for Recombinant Mouse MDH2 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse MDH2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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