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Recombinant Human MED21 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23221

Novus Biologicals, part of Bio-Techne
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NBP2-23221

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-144 of Human MED21

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADRLTQ LQDAVNSLAD QFCNAIGVLQ QCGPPASFNN IQTAINKDQP ANPTEEYAQL FAALIARTAK DIDVLIDSLP SEESTAALQA ASLYKLEEEN HEAATCLEDV VYRGDMLLEK IQSALADIAQ SQLKTRSGTH SQSLPDS

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MED21 His Protein

SDS-PAGE: Recombinant Human MED21 His Protein [NBP2-23221]

SDS-PAGE: Recombinant Human MED21 His Protein [NBP2-23221]

SDS-Page: Recombinant Human MED21 His Protein [NBP2-23221] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP2-23221
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MED21

MED21 is a member of the mediator complex subunit 21 family. The protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. Recombinant human MED21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

hSrb7, mediator complex subunit 21SRB7 (suppressor of RNA polymerase B, yeast) homolog, RNA polymerase II complex component SRB7, RNA polymerase II holoenzyme component SRB7, RNAPII complex component SRB7, SRB7 suppressor of RNA polymerase B homolog, SRB7SRB7 suppressor of RNA polymerase B homolog (yeast), SURB7mediator of RNA polymerase II transcription subunit 21

Gene Symbol

MED21

Additional MED21 Products

Product Documents for Recombinant Human MED21 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human MED21 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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