Recombinant Human Methionine Sulfoxide Reductase B His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49302

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Methionine Sulfoxide Reductase B Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-116 of Human Methionine Sulfoxide Reductase B

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSFCSFFGGE VFQNHFEPGV YVCAKCGYEL FSSRSKYAHS SPWPAFTETI HADSVAKRPE HNRSEALKVS CGKCGNGLGH EFLNDGPKPG QSRFCIFSSS LKFVPKGKET SASQGH

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

14.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Methionine Sulfoxide Reductase B His Protein

SDS-PAGE: Recombinant Human Methionine Sulfoxide Reductase B His Protein [NBP1-49302]

SDS-PAGE: Recombinant Human Methionine Sulfoxide Reductase B His Protein [NBP1-49302]

SDS-Page: Recombinant Human Methionine Sulfoxide Reductase B Protein [NBP1-49302] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-49302
Formulation 20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 0.1 mM PMSF, 2 mM EDTA, 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Methionine Sulfoxide Reductase B

SEPX1, also known as Methionine sulfoxide reductase B1 (MSRB1), is a selenoprotein. Methionine sulfoxide reductases(MSRs) catalyze reduction of free and protein-bound methionine sulfoxides to corresponding methionines. The oxidation of methionine by ROS generates a diastereomeric mixture of methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO). Two distinct enzyme families evolved for reduction of these sulfoxides, with methionine-S-sulfoxide reductase (MsrA) being stereospecific for Met-S-SO and methionine-R-sulfoxide reductase (MsrB) for Met-R-SO. In bacteria, the selenocystein (Sec/U) element is located immediately following the UGA codon within the reading frame for the selenoprotein so we mutated Sec-95 to Cys. Recombinant human SEPX1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

EC 1.8.4.-, methionine sulfoxide reductase, methionine-R-sulfoxide reductase B1, MGC3344, MsrB1, MSRB1selenoprotein R, Selenoprotein X, selenoprotein X, 1, SELR, SelX

Gene Symbol

MSRB1

Additional Methionine Sulfoxide Reductase B Products

Product Documents for Recombinant Human Methionine Sulfoxide Reductase B His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Methionine Sulfoxide Reductase B His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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