Recombinant Human MIF His Avi-tag Protein [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18522
Key Product Details
Source
E. coli
Tag
His, Avi-tag
Conjugate
Biotin
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A single, non-glycosylated, biotinylated polypeptide chain containing 136 amino acids corresponding to human MIF, with 6x His and Avi tags at the C-terminus. Source: E. coli
Amino Acid Sequence: MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFAHHHHH HGLNDIFEAQ KIEWHE
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug, as determined by LAL method.
Predicted Molecular Mass
15.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human MIF His Avi-tag Protein [Biotin]
![SDS-PAGE: Recombinant Human MIF His Avi-tag Protein [Biotin] [NBP3-18522]](https://resources.bio-techne.com/images/products/Recombinant Human MIF His Avi-tag Protein [Biotin]-SDS-Page-NBP3-18522-img0001.jpg "SDS-PAGE: Recombinant Human MIF His Avi-tag Protein [Biotin] [NBP3-18522]")
SDS-PAGE: Recombinant Human MIF His Avi-tag Protein [Biotin] [NBP3-18522]
SDS-Page: Recombinant Human MIF His Avi-tag Protein [Biotin] [NBP3-18522] - Western blot under reducing and non-reducing conditionsFormulation, Preparation and Storage
NBP3-18522
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0, 0.02 % Tween-20. |
| Preservative | 5% Trehalose |
| Concentration | Lyoph |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: MIF
Long Name
Macrophage Migration Inhibitory Factor
Alternate Names
EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase
Gene Symbol
MIF
Additional MIF Products
Product Documents for Recombinant Human MIF His Avi-tag Protein [Biotin]
Product Specific Notices for Recombinant Human MIF His Avi-tag Protein [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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