Recombinant Human MST2/STK3 His Protein

Name: Recombinant Human MST2/STK3 His Protein
Brand: Novus Biologicals
SKU: NBP2-53049

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53049

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Baculovirus

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all MST2/STK3 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-322 of Human MST2/STK3

Source: Baculovirus

Amino Acid Sequence: ADPMEQPPAP KSKLKKLSED SLTKQPEEVF DVLEKLGEGS YGSVFKAIHK ESGQVVAIKQ VPVESDLQEI IKEISIMQQC DSPYVVKYYG SYFKNTDLWI VMEYCGAGSV SDIIRLRNKT LIEDEIATIL KSTLKGLEYL HFMRKIHRDI KAGNILLNTE GHAKLADFGV AGQLTDTMAK RNTVIGTPFW MAPEVIQEIG YNCVADIWSL GITSIEMAEG KPPYADIHPM RAIFMIPTNP PPTFRKPELW SDDFTDFVKK CLVKNPEQRA TATQLLQHPF IKNAKPVSIL RDLITEAMEI KAKRHEEQQR ELEEEEENSD EDELDHHHHH H

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

37.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MST2/STK3 His Protein

SDS-PAGE: Recombinant Human MST2/STK3 His Protein [NBP2-53049]

SDS-Page: MST2/STK3 Recombinant Protein [NBP2-53049] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP2-53049

Formulation	PBS (pH 7.4), 10% glycerol
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MST2/STK3

Mammalian Sterile 20-Like 2 (Mst2) is most similar to the previously identified Mst1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in-vitro kinase assay indicates that Mst2 can phosphorylate an exogenous substrate, as well as itself, and phospho-amino-acid analysis indicates that it is a serine/threonine protein kinase (1) Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2. Results indicate that Mst2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway (2). When stably expressed in HeLa cells, Mst highly sensitizes the cells to death receptor-mediated apoptosis by accelerating caspase-3 activation. These findings suggest that Mst1 and Mst2 play a role in apoptosis both upstream and downstream of caspase activation (3).

Alternate Names

KRS1, Mess1, MST3, STK3

Gene Symbol

STK3

Additional MST2/STK3 Products

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COA

Product Specific Notices for Recombinant Human MST2/STK3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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