Skip to main content

Recombinant Human Myeloid leukemia factor 1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50985

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP1-50985-0.1mg
NBP1-50985-0.5mg

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-268 of Human Myeloid leukemia factor 1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MFRMLNSSFE DDPFFSESIL AHRENMRQMI RSFSEPFGRD LLSISDGRGR AHNRRGHNDG EDSLTHTDVS SFQTMDQMVS NMRNYMQKLE RNFGQLSVDP NGHSFCSSSV MTYSKIGDEP PKVFQASTQT RRAPGGIKET RKAMRDSDSG LEKMAIGHHI HDRAHVIKKS KNKKTGDEEV NQEFINMNES DAHAFDEEWQ SEVLKYKPGR HNLGNTRMRS VGHENPGSRE LKRREKPQQS PAIEHGRRSN VLGDKLHIKG SSVKSNKK

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

32.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Myeloid leukemia factor 1 His Protein

SDS-PAGE: Recombinant Human Myeloid leukemia factor 1 His Protein [NBP1-50985]

SDS-PAGE: Recombinant Human Myeloid leukemia factor 1 His Protein [NBP1-50985]

SDS-Page: Recombinant Human Myeloid leukemia factor 1 Protein [NBP1-50985] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-50985
Formulation 20 mM Tris-HCl buffer (pH 8.0), 40% glycerol, 5mM DTT, 200 mM NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myeloid leukemia factor 1

MLF1 (Myeloid leukemia factor 1) belongs to MLF family, and is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. This protein interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 normally functions in multi-potent progenitor cells, and its dysregulation may be somewhat responsible for leukemogenesis. Recombinant human MLF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Myelodysplasia-myeloid leukemia factor 1, myeloid leukemia factor 1

Gene Symbol

MLF1

Additional Myeloid leukemia factor 1 Products

Product Documents for Recombinant Human Myeloid leukemia factor 1 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Myeloid leukemia factor 1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...