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Recombinant Human MYL5 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72469

Novus Biologicals, part of Bio-Techne
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NBP1-72469-250ug
NBP1-72469-50ug

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-173 of Human MYL5

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASRKT KKKEGGALRA QRASSNVFSN FEQTQIQEFK EAFTLMDQNR DGFIDKEDLK DTYASLGKTN VKDDELDAML KEASGPINFT MFLNLFGEKL SGTDAEETIL NAFKMLDPDG KGKINKEYIK RLLMSQADKM TAEEVDQMFQ FASIDVAGNL DYKALSYVIT HGEEKEE

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

22.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MYL5 His Protein

SDS-PAGE: Recombinant Human MYL5 His Protein [NBP1-72469]

SDS-PAGE: Recombinant Human MYL5 His Protein [NBP1-72469]

SDS-Page: Recombinant Human MYL5 Protein [NBP1-72469] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72469
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT, 0.1 mM PMSF
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MYL5

Myosin regulatory light chain 5, also known as MYL5, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. Reconstitution of myosin with regulatory light chain 5 or alkali light chain increases filament velocity to intermediate rates, and readdition of both classes of light chains fully restores the original sliding velocity. Recombinant human MYL5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

light polypeptide 5, regulatory, MYLC2, myLC-2, myosin light chain 5, Myosin regulatory light chain 5, myosin, light chain 5, regulatory, Superfast myosin regulatory light chain 2

Gene Symbol

MYL5

Additional MYL5 Products

Product Documents for Recombinant Human MYL5 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human MYL5 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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