Recombinant Human NCALD His Protein

Name: Recombinant Human NCALD His Protein
Brand: Novus Biologicals
SKU: NBP1-50975

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50975

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all NCALD Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-193 of Human NCALD

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NCALD His Protein

SDS-PAGE: Recombinant Human NCALD His Protein [NBP1-50975]

SDS-Page: Recombinant Human NCALD Protein [NBP1-50975] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-50975

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 40% glycerol, 100 mM NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCALD

Neurocalcin-delta, also known NCALD, is a member of the neuronal calcium sensor (NCS) family. NCALD is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. This protein is thought to be a regulator of signal transduction by G protein-coupled receptor. Recombinant human NCALD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

MGC33870, MGC74858, neurocalcin delta, neurocalcin-delta

Gene Symbol

NCALD

Additional NCALD Products

Product Documents for Recombinant Human NCALD His Protein

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COA

Product Specific Notices for Recombinant Human NCALD His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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