Recombinant Human NDUFAF1 His Protein

Name: Recombinant Human NDUFAF1 His Protein
Brand: Novus Biologicals
SKU: NBP2-23263

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23263

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all NDUFAF1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-327 of Human NDUFAF1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSYPFLGIR FAEYSSSLQK PVASPGKASS QRKTEGDLQG DHQKEVALDI TSSEEKPDVS FDKAIRDEAI YHFRLLKDEI VDHWRGPEGH PLHEVLLEQA KVVWQFRGKE DLDKWTVTSD KTIGGRSEVF LKMGKNNQSA LLYGTLSSEA PQDGESTRSG YCAMISRIPR GAFERKMSYD WSQFNTLYLR VRGDGRPWMV NIKEDTDFFQ RTNQMYSYFM FTRGGPYWQE VKIPFSKFFF SNRGRIRDVQ HELPLDKISS IGFTLADKVD GPFFLEIDFI GVFTDPAHTE EFAYENSPEL NPRLFK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NDUFAF1 His Protein

SDS-PAGE: Recombinant Human NDUFAF1 His Protein [NBP2-23263]

SDS-Page: NDUFAF1 Protein [NBP2-23263]

Formulation, Preparation and Storage

NBP2-23263

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 40% glycerol, 1 mM DTT, 2 mM EDTA
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDUFAF1

NDUFAF1 is a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The protein is required for assembly of complex I, and mutations in this protein are a cause of mitochondrial complex I deficiency. Recombinant human NDUFAF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CGI-65, CIA30CGI65, complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1

Gene Symbol

NDUFAF1

Additional NDUFAF1 Products

Product Documents for Recombinant Human NDUFAF1 His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human NDUFAF1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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