Recombinant Human NSMCE1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23292

Novus Biologicals, part of Bio-Techne
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NBP2-23292
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all NSMCE1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-266 of Human NSMCE1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMQGSTRR MGVMTDVHRR FLQLLMTHGV LEEWDVKRLQ THCYKVHDRN ATVDKLEDFI NNINSVLESL YIEIKRGVTE DDGRPIYALV NLATTSISKM ATDFAENELD LFRKALELII DSETGFASST NILNLVDQLK GKKMRKKEAE QVLQKFVQNK WLIEKEGEFT LHGRAILEME QYIRETYPDA VKICNICHSL LIQGQSCETC GIRMHLPCVA KYFQSNAEPR CPHCNDYWPH EIPKVFDPEK ERESGVLKSN KKSLRSRQH

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NSMCE1 His Protein

SDS-PAGE: Recombinant Human NSMCE1 His Protein [NBP2-23292]

SDS-PAGE: Recombinant Human NSMCE1 His Protein [NBP2-23292]

SDS-Page: NSMCE1 Protein [NBP2-23292]

Formulation, Preparation and Storage

NBP2-23292
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NSMCE1

NSMCE1 is a component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). NSMCE1 has in vitro ubiquitin ligase activity in presence of NDNL2. Recombinant human NSMCE1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Non-SMC element 1 homolog, non-SMC element 1 homolog (S. cerevisiae), non-structural maintenance of chromosomes element 1 homolog, NSE1

Gene Symbol

NSMCE1

Additional NSMCE1 Products

Product Documents for Recombinant Human NSMCE1 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human NSMCE1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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