Recombinant E. coli NusA Protein

Name: Recombinant E. coli NusA Protein
Brand: Novus Biologicals
SKU: NBC1-22923

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-22923

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all NusA Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 1-495 of E.coli NusA

Source: E.coli

Amino Acid Sequence: MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVQID RKSGDFDTFR RWLVVDEVTQ PTKEITLEAA RYEDESLNLG DYVEDQIESV TFDRITTQTA KQVIVQKVRE AERAMVVDQF REHEGEIITG VVKKVNRDNI SLDLGNNAEA VILREDMLPR ENFRPGDRVR GVLYSVRPEA RGAQLFVTRS KPEMLIELFR IEVPEIGEEV IEIKAAARDP GSRAKIAVKT NDKRIDPVGA CVGMRGARVQ AVSTELGGER IDIVLWDDNP AQFVINAMAP ADVASIVVDE DKHTMDIAVE AGNLAQAIGR NGQNVRLASQ LSGWELNVMT VDDLQAKHQA EAHAAIDTFT KYLDIDEDFA TVLVEEGFST LEELAYVPMK ELLEIEGLDE PTVEALRERA KNALATIAQA QEESLGDNKP ADDLLNLEGV DRDLAFKLAA RGVCTLEDLA EQGIDDLADI EGLTDEKAGA LIMAARNICW FGDEA

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli NusA Protein

SDS-PAGE: Recombinant E. coli NusA Protein [NBC1-22923]

SDS-Page: NusA Protein [NBC1-22923] - NusA, 54 kDa (495 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-22923

Formulation	PBS (pH 7.4)
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NusA

NusA is a key component in both Prevention and enhancement of transcriptional termination. It is important in both Rho-dependent and intrinsic termination, as well as in lambda and other phage antitermination systems. The gene was first identified by isolation of the nusAl mutation, which restricts bacteriophage l growth by preventing the antitermination activity of the l N protein. NusA is involved in transcriptional antitermination in the cell. It has been shown to specifically aid in read-through of the RNA polymerase genes rpoB and rpoC, as well as in successful synthesis of the ribosomal RNA genes. Recombinant NusA was expressed in E.coli and purified by using conventional chromatography techniques

Alternate Names

ECK3158, JW3158, Transcription termination/antitermination L factor

Additional NusA Products

Product Documents for Recombinant E. coli NusA Protein

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COA

Product Specific Notices for Recombinant E. coli NusA Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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