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Recombinant Human p21/CIP1/CDKN1A His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22976

Novus Biologicals, part of Bio-Techne
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NBP2-22976
NBP2-22976-100ug

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-164 of Human p21/CIP1/CDKN1A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSEPAGDVRQ NPCGSKACRR LFGPVDSEQL SRDCDALMAG CIQEARERWN FDFVTETPLE GDFAWERVRG LGLPKLYLPT GPRRGRDELG GGRRPGTSPA LLQGTAEEDH VDLSLSCTLV PRSGEQAEGS PGGPGDSQGR KRRQTSMTDF YHSKRRLIFS KRKP

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

20.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human p21/CIP1/CDKN1A His Protein

SDS-PAGE: Recombinant Human p21/CIP1/CDKN1A His Protein [NBP2-22976]

SDS-PAGE: Recombinant Human p21/CIP1/CDKN1A His Protein [NBP2-22976]

SDS-Page: Recombinant Human p21/CIP1/CDKN1A Protein [NBP2-22976]

Formulation, Preparation and Storage

NBP2-22976
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 10% glycerol, 0.1 M NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p21/CIP1/CDKN1A

CDKN1A, also known as P21 or WAF1, plays a critical role in the cellular response to DNA damage, and its overexpression results in cell cycle arrest. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, CDKN1A may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. This protein may be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Recombinant human CDKN1A protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

p21 Cyclin Dependent Kinase 4 Inhibitor 1A

Alternate Names

CDKN1A, CIP1

Gene Symbol

CDKN1A

Additional p21/CIP1/CDKN1A Products

Product Documents for Recombinant Human p21/CIP1/CDKN1A His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human p21/CIP1/CDKN1A His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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