Recombinant Mouse PBEF/Visfatin/NAMPT His Protein

Name: Recombinant Mouse PBEF/Visfatin/NAMPT His Protein
Brand: Novus Biologicals
SKU: NBC1-22597

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-22597

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all PBEF/Visfatin Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1- 491 of Mouse PBEF/Visfatin/NAMPT

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKVR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKEVYREH FQDDVFNERG WNYILEKYDG HLPIEVKAVP EGSVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KKWSIENVSF GSGGALLQKL TRDLLNCSFK CSYVVTNGLG VNVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGHDLLHT VFKNGKVTKS YSFDEVRKNA QLNIEQDVAP H

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

57.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse PBEF/Visfatin/NAMPT His Protein

SDS-PAGE: Recombinant Mouse PBEF/Visfatin/NAMPT His Protein [NBC1-22597]

SDS-Page: Recombinant Mouse PBEF/Visfatin/NAMPT Protein [NBC1-22597] - Visfatin, 57 kDa (511 aa) , confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-22597

Formulation	PBS (pH 7.4)
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PBEF/Visfatin

Visfatin is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (pre-B cell colony-enhancing factor, PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor. Recombinant mouse visfatin was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Alternate Names

NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin

Gene Symbol

NAMPT

Additional PBEF/Visfatin Products

Product Documents for Recombinant Mouse PBEF/Visfatin/NAMPT His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Mouse PBEF/Visfatin/NAMPT His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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