Recombinant Human PDE7B GST (N-Term) Protein

Name: Recombinant Human PDE7B GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00027115-P01

Novus Biologicals, part of Bio-Techne | Catalog # H00027115-P01

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Affinity Purification, Microarray, SDS-PAGE, Western Blot

View all PDE7B Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-450 of Human PDE7B

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MSCLMVERCGEILFENPDQNAKCVCMLGDIRLRGQTGVRAERRGSYPFIDFRLLNSTTYSGEIGTKKKVKRLLSFQRYFHASRLLRGIIPQAPLHLLDEDYLGQARHMLSKVGMWDFDIFLFDRLTNGNSLVTLLCHLFNTHGLIHHFKLDMVTLHRFLVMVQEDYHSQNPYHNAVHAADVTQAMHCYLKEPKLASFLTPLDIMLGLLAAAAHDVDHPGVNQPFLIKTNHHLANLYQNMSVLENHHWRSTIGMLRESRLLAHLPKEMTQDIEQQLGSLILATDINRQNEFLTRLKAHLHNKDLRLEDAQDRHFMLQIALKCADICNPCRIWEMSKQWSERVCEEFYRQGELEQKFELEISPLCNQQKDSIPSIQIGFMSYIVEPLFREWAHFTGNSTLSENMLGHLAHNKAQWKSLLPRQHRSRGSSGSGPDHDHAGQGTESEEQEGDSP

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

78.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PDE7B GST (N-Term) Protein

SDS-PAGE: Recombinant Human PDE7B GST (N-Term) Protein [H00027115-P01]

SDS-Page: Recombinant Human PDE7B Protein [H00027115-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Formulation, Preparation and Storage

H00027115-P01

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: PDE7B

Phosphodiesterases (PDEs) regulate intracellular levels of cAMP and cGMP, cyclic nucleotides that play important roles as second messengers in multiple physiologic processes. PDE7B belongs to the large superfamily of PDEs, which can be distinguished by their structure, tissue expression, localization, substrate specificity, regulation, and sensitivity to PDE inhibitors (Hetman et al., 2000 [PubMed 10618442]).[supplied by OMIM]

Long Name

cAMP-specific 3',5'-cyclic phosphodiesterase 7B

Alternate Names

bA472E5.1, cAMP-specific 3'-5'-cyclic phosphodiesterase 7B, EC 3.1.4, EC 3.1.4.17, high-affinity cAMP-specific 3'-5'-cyclic phosphodiesterase, MGC88256, phosphodiesterase 7B, rolipram-insensitive phosphodiesterase type 7

Gene Symbol

PDE7B

Additional PDE7B Products

Product Documents for Recombinant Human PDE7B GST (N-Term) Protein

Product Datasheets

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COA

SDS

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Product Specific Notices for Recombinant Human PDE7B GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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