Recombinant Human PGAM1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52620

Novus Biologicals, part of Bio-Techne
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NBP2-52620
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Key Product Details

Source

E. coli

Accession #

NP_002620

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all PGAM1 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-254 of Human PGAM1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

30.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is >300 units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PGAM1 His Protein

SDS-PAGE: Recombinant Human PGAM1 His Protein [NBP2-52620]

SDS-PAGE: Recombinant Human PGAM1 His Protein [NBP2-52620]

SDS-Page: PGAM1 Recombinant Protein [NBP2-52620]

Formulation, Preparation and Storage

NBP2-52620
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PGAM1

PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in this protein cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

BPG-dependent PGAM 1, EC 3.1.3.13, EC 5.4.2.1, EC 5.4.2.4, PGAMA, PGAM-Bphosphoglycerate mutase 1, phosphoglycerate mutase 1 (brain), phosphoglycerate mutase A, nonmuscle form, Phosphoglycerate mutase isozyme B

Gene Symbol

PGAM1

UniProt

NP_002620 (Human)

Additional PGAM1 Products

Product Documents for Recombinant Human PGAM1 His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human PGAM1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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