Recombinant Human Phosphopantothenate-cysteine ligase His Protein

Name: Recombinant Human Phosphopantothenate-cysteine ligase His Protein
Brand: Novus Biologicals
SKU: NBP1-78806

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78806

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Phosphopantothenate-cysteine ligase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-311 of Human Phosphopantothenate-cysteine ligase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAEMDPVAEF PQPPGAARWA EVMARFAARL GAQGRRVVLV TSGGTKVPLE ARPVRFLDNF SSGRRGATSA EAFLAAGYGV LFLYRARSAF PYAHRFPPQT WLSALRPSGP ALSGLLSLEA EENALPGFAE ALRSYQEAAA AGTFLAVEFT TLADYLHLLQ AAAQALNPLG PSAMFYLAAA VSDFYVPVSE MPEHKIQSSG GPLQITMKMV PKLLSPLVKD WAPKAFIISF KLETDPAIVI NRARKALEIY QHQVVVANIL ESRQSFVFIV TKDSETKLLL SEEEIEKGVE IEEKIVDNLQ SRHTAFIGDR N

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

36.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Phosphopantothenate-cysteine ligase His Protein

SDS-PAGE: Recombinant Human Phosphopantothenate-cysteine ligase His Protein [NBP1-78806]

SDS-Page: Recombinant Human Phosphopantothenate-cysteine ligase Protein [NBP1-78806] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-78806

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phosphopantothenate-cysteine ligase

PPCS, also known as phosphopantothenate--cysteine ligase, catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. Recombinant human PPCS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

FLJ11838, MGC117357, phosphopantothenoylcysteine synthetase, PPC synthetase

Gene Symbol

PPCS

Additional Phosphopantothenate-cysteine ligase Products

Product Documents for Recombinant Human Phosphopantothenate-cysteine ligase His Protein

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COA

Product Specific Notices for Recombinant Human Phosphopantothenate-cysteine ligase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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