Recombinant E. coli PKLR His Protein

Name: Recombinant E. coli PKLR His Protein
Brand: Novus Biologicals
SKU: NBP2-22656

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22656

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all PKLR Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-470 of E.coli PKLR

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENK GVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIREH LKAHGGENIH IISKIENQEG LNNFDEILEA SDGIMVARGD LGVEIPVEEV IFAQKMMIEK CIRARKVVIT ATQMLDSMIK NPRPTRAEAG DVANAILDGT DAVMLSGESA KGKYPLEAVS IMATICERTD RVMNSRLEFN NDNRKLRITE AVCRGAVETA EKLDAPLIVV ATQGGKSARA VRKYFPDATI LALTTNEKTA HQLVLSKGVV PQLVKEITST DDFYRLGKEL ALQSGLAHKG DVVVMVSGAL VPSGTTNTAS VHVL

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

53.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli PKLR His Protein

SDS-PAGE: Recombinant E. coli PKLR His Protein [NBP2-22656]

SDS-Page: Pyruvate Kinase Protein [NBP2-22656]

Formulation, Preparation and Storage

NBP2-22656

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKLR

pyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. Recombinant E.coli pykF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Pyruvate Kinase, Liver And RBC

Alternate Names

PK1, PKL, Pyruvate Kinase 1, RPK

Gene Symbol

PKLR

Additional PKLR Products

Product Documents for Recombinant E. coli PKLR His Protein

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COA

Product Specific Notices for Recombinant E. coli PKLR His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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