Recombinant Human Pleiotrophin/PTN Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35233
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 136 amino acids corresponding to Pleiotrophin/PTN Source: E. coli
Uniprot ID: P21246
Amino Acid Sequence: GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of Pleiotrophin/PTN as determined by LAL method.
Predicted Molecular Mass
15.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Pleiotrophin/PTN protein is fully biologically active when compared to standard. The biological activity was measured by its ability to enhance neurite outgrowth of E16E18 rat embryonic cortical neurons, when neurons were plated on 96 well culture plates that had been precoated with 100 ul/well of a solution of 5-10 ug/ml rHuPTN.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human Pleiotrophin/PTN Protein
![SDS-PAGE: Recombinant Human Pleiotrophin/PTN Protein [NBP2-35233]](https://resources.bio-techne.com/images/products/Human-Pleiotrophin-PTN-Protein-SDS-Page-NBP2-35233-img0001.jpg "SDS-PAGE: Recombinant Human Pleiotrophin/PTN Protein [NBP2-35233]")
SDS-PAGE: Recombinant Human Pleiotrophin/PTN Protein [NBP2-35233]
SDS-Page: Human Pleiotrophin/PTN Protein [NBP2-35233]Formulation, Preparation and Storage
NBP2-35233
| Formulation | Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Pleiotrophin/PTN
Additional Pleiotrophin/PTN Products
Product Documents for Recombinant Human Pleiotrophin/PTN Protein
Product Specific Notices for Recombinant Human Pleiotrophin/PTN Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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