Recombinant Mouse PRMT1 His-MBP Protein

Name: Recombinant Mouse PRMT1 His-MBP Protein
Brand: Novus Biologicals
SKU: NBC1-18445

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18445

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His-MBP

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all PRMT1 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-MBP tag and corresponding to the amino acids 1-353 of mouse PRMT1

Source: E.coli

Amino Acid Sequence: MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 30 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse PRMT1 His-MBP Protein

SDS-PAGE: Recombinant Mouse PRMT1 His-MBP Protein [NBC1-18445]

SDS-Page: PRMT1 Protein [NBC1-18445] - PRMT1 isoform2, 84 kDa (750 aa) with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-18445

Formulation	40mM Tris-HCl buffer (pH 8.0), 100 mM NaCl, 4mM MgCl2, 2 mM DTT, 40% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRMT1

Protein arginine N-methyltransferase 1(PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins. Mouse PRMT1(AAF37293) shares 99.9% sequence identity with the human proteins(NP_938074). Recombinant mouse PRMT1, fused to His-MBP tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Protein Arginine Methyltransferase 1

Alternate Names

ANM1, HCP1, HMT1, HMT2, HRMT1L2, IR1B4

Gene Symbol

PRMT1

Additional PRMT1 Products

Product Documents for Recombinant Mouse PRMT1 His-MBP Protein

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COA

Product Specific Notices for Recombinant Mouse PRMT1 His-MBP Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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