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Recombinant Human PU.1/Spi-1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-51976

Novus Biologicals, part of Bio-Techne
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NBP2-51976-0.05mg
NBP2-51976-0.25mg

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-271 of Human PU.1/Spi-1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLQACKM EGFPLVPPQP SEDLVPYDTD LYQRQTHEYY PYLSSDGESH SDHYWDFHPH HVHSEFESFA ENNFTELQSV QPPQLQQLYR HMELEQMHVL DTPMVPPHPS LGHQVSYLPR MCLQYPSLSP AQPSSDEEEG ERQSPPLEVS DGEADGLEPG PGLLPGETGS KKKIRLYQFL LDLLRSGDMK DSIWWVDKDK GTFQFSSKHK EALAHRWGIQ KGNRKKMTYQ KMARALRNYG KTGEVKKVKK KLTYQFSGEV LGRGGLAERR HPPH

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

33.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PU.1/Spi-1 His Protein

SDS-PAGE: Recombinant Human PU.1/Spi-1 His Protein [NBP2-51976]

SDS-PAGE: Recombinant Human PU.1/Spi-1 His Protein [NBP2-51976]

SDS-Page: PU.1/Spi-1 Recombinant Protein [NBP2-51976] - 15% SDS Page (3 ug)

Formulation, Preparation and Storage

NBP2-51976
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PU.1/Spi-1

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Hematopoietic transcription factor PU.1

Alternate Names

OF, SFPI1, Spi-1, SPI-A, SPI1

Gene Symbol

SPI1

Additional PU.1/Spi-1 Products

Product Documents for Recombinant Human PU.1/Spi-1 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human PU.1/Spi-1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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