Do you have an explanation why the 26kDa protein is running at 52kDa? Are there any indices for dimers?

Name: Recombinant Human R3HDML GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00140902-P01

This particular protein is distributed by us for a Taiwanese company called Abnova. Abnova adds a GST tag to their recombinant proteins. This GST tag will add approximately 26kDa in size to the protein it is attached to.

Recombinant Human R3HDML GST (N-Term) Protein

Novus Biologicals, part of Bio-Techne | Catalog # H00140902-P01

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Product Datasheet / COA / SDS

Discontinued Product

H00140902-P01 has been discontinued. View all R3HDML products.

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Affinity Purification, Microarray, Western Blot

View all R3HDML Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-253 of Human R3HDML

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MPLLPSTVGLAGLLFWAGQAVNALIMPNATPAPAQPESTAMRLLSGLEVPRYRRKRHISVRDMNALLDYHNHIRASVYPPAANMEYMVWDKRLARAAEAWATQCIWAHGPSQLMRYVGQNLSIHSGQYRSVVDLMKSWSEEKWHYLFPAPRDCNPHCPWRCDGPTCSHYTQMVWASSNRLGCAIHTCSSISVWGNTWHRAAYLVCNYAIKGNWIGESPYKMGKPCSSCPPSYQGSCNSNMCFKGLKSNKFTWF

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human R3HDML GST (N-Term) Protein

12.5% SDS-PAGE Stained with Coomassie Blue.

Formulation, Preparation and Storage

H00140902-P01

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: R3HDML

R3HDML, also known as R3H Domain Containing-Like, is a 29kDa 253 amino acid protein. R3HDML is a secreted protein that belongs to the CRISP family.

Alternate Names

Cysteine-rich secretory protein R3HDML, dJ881L22.3, MGC129564, peptidase inhibitor R3HDML, R3H domain (binds single-stranded nucleic acids) containing-like, R3H domain containing-like

Gene Symbol

R3HDML

Additional R3HDML Products

Product Documents for Recombinant Human R3HDML GST (N-Term) Protein

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Recombinant Human R3HDML GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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