I am looking to purchase recombinant EPAC protein, hopefully EPAC1 but EPAC2 will work as well. I need it for in vitro studies where I am measuring its ability to activate RAP in the presence of various compounds. I see that you sell recombinant EPAC2 (H00011069-P01) and EPAC1 (H00010411-P01) and was wondering if these samples would work for my experiments?

Name: Recombinant Human RAPGEF3 GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00010411-P01

The catalog #s which you selected are for the products that we distribute for a Taiwanese company called Abnova. Their R&D team grows all of their proteins in a cell free wheat germ system. Because of this particular system, some of their proteins may not follow normal PTM or protein folding processing. We therefore generally do not recommend them for use in experiments that require protein activity. These are the only Epac1 and Epac 2 proteins that we carry, and they may work in your particular experiment, but we would not be able to guarantee results.

Recombinant Human RAPGEF3 GST (N-Term) Protein

Novus Biologicals, part of Bio-Techne | Catalog # H00010411-P01

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Product Datasheet / COA / SDS

Discontinued Product

H00010411-P01 has been discontinued. View all RAPGEF3 products.

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Affinity Purification, Microarray, SDS-PAGE, Western Blot

View all RAPGEF3 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-881 of Human RAPGEF3

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MVLRRMHRPRSCSYQLLLEHQRPSCIQGLRWTPLTNSEESLDFSESLEQASTERVLRAGRQLHRHLLATCPNLIRDRKYHLRLYRQCCSGRELVDGILALGLGVHSRSQVVGICQVLLDEGALCHVKHDWAFQDRDAQFYRFPGPEPEPVGTHEMEEELAEAVALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERASQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMGPDSSAHDPTETFLSDFLLTHRVFMPSAQLCAALLHHFHVEPAGGSEQERSTYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALAKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

125.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RAPGEF3 GST (N-Term) Protein

SDS-PAGE: Recombinant Human RAPGEF3 GST (N-Term) Protein [H00010411-P01]

SDS-Page: Recombinant Human RAPGEF3 Protein [H00010411-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Formulation, Preparation and Storage

H00010411-P01

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: RAPGEF3

RAPGEF3, also known as Rap guanine nucleotide exchange factor 3, is a 104 kDa 923 amino acid protein with two shorter isoforms produced by alternative splicing. The gene is necessary for rearranging actin at cell to cell junctions. RAPGEF3 is currently being studied for its involvement with leukemia, melanoma, pancreatitis, thyroiditis, carcinoma, prostatitis and neuronitis. RAPGEF3 is linked to the chemotaxis, cell adhesion, insulin secretion, and immune system pathways where it interacts with GNAI2, GNAI1, PDE3B, RAP1A and RAP1B.

Long Name

Rap guanine nucleotide exchange factor 3

Alternate Names

cAMP-GEFI, CGEF1, EPAC, EPAC 1, EPAC1

Gene Symbol

RAPGEF3

Additional RAPGEF3 Products

Product Documents for Recombinant Human RAPGEF3 GST (N-Term) Protein

Product Datasheets

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COA

SDS

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Product Specific Notices for Recombinant Human RAPGEF3 GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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