Recombinant Mouse Rapsyn His Protein

Name: Recombinant Mouse Rapsyn His Protein
Brand: Novus Biologicals
SKU: NBP2-23403

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23403

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Rapsyn Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-412 of Mouse Rapsyn

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGQDQTK QQIEKGLQLY QSNQTEKALQ VWMKVLEKGS DLVGRFRVLG CLVTAHSEMG RYKEMLKFAV VQIDTARGLE DADFLLESYL NLARSNEKLC EFHKTISYCK TCLGLPGTRA GAQLGGQVSL SMGNAFLGLS LFQKALESFE KALRYAHNND DTMLECRVCC SLGSFYAQVK DYEKALFFPC KAAELVNDYG KGWSLKYRAM SQYHMAVAYR LLGHLGSAME CCEESMKIAL QHGDRPLQAL CLLCFADIHR SRGDLETAFP RYDSAMSIMT EIGNRLGQVH VLLGVAKCWM ARKVQDKALD AIEKAQDLAE EVGNKLSQLK LHCLSESIYR SKGLQRELRT HVVRFHECVE ETELYCGLCG ESIGERNSRL QALPCSHIFH LRCLQNNGTR SCPNCRRSSM KPGFV

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Rapsyn His Protein

SDS-PAGE: Recombinant Mouse Rapsyn His Protein [NBP2-23403]

SDS-Page: Recombinant Mouse Rapsyn Protein [NBP2-23403] - 15% SDS-Page (3ug)

Formulation, Preparation and Storage

NBP2-23403

Formulation	20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rapsyn

43 kDa receptor-associated protein of the synapse, also known as RAPSN, is expressed in the postsynaptic membrane of skeletal muscle. RAPSN is required for the clustering of nicotinic acetylcholine receptors (nAChR). It self-associates through at least two of its seven tetra-tricopeptide repeats (TPRs). RAPSN interacts with the large intracellular domain of the nAChR a subunit through the hydrophobic surface of the coiled-coil domain. This protein modifies trafficking of AChR within the cell. Overexpression inhibits agrin-induced AChR clustering pathway. Recombinant human RAPSN protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

43 kDa postsynaptic protein, Acetylcholine receptor-associated 43 kDa protein, CMS1D43 kDa receptor-associated protein of the synapse, CMS1EMGC3597, RAPsyn, receptor-associated protein of the synapse, receptor-associated protein of the synapse, 43kD, RNF205RING finger protein 205

Gene Symbol

RAPSN

Additional Rapsyn Products

Product Documents for Recombinant Mouse Rapsyn His Protein

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COA

Product Specific Notices for Recombinant Mouse Rapsyn His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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