Recombinant Botulinum Neurotoxin Type A Light Chain, CF
R&D Systems, part of Bio-Techne | Catalog # 4489-ZN
Key Product Details
Product Specifications
Source
E. coli-derived c. botulinum BoNT-A Light Chain protein
Met1-Phe425, with an N-terminal Met and 6-His tag
Met1-Phe425, with an N-terminal Met and 6-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.1 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
50 kDa
SDS-PAGE
52 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, SNAPtide.
The specific activity is >1 pmol/min/µg, as measured under the described conditions.
The specific activity is >1 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4489-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Tween® 20. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: BoNT-A Light Chain
Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2-4). E. coli-expressed recombinant light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.
References
- Willems, A. et al. (1993) Res. Microbiol. 144:547.
- Montecucco, C. and Giampietro, S. (1993) Trends. Biochem. Sci. 18:324.
- Turton, K., et al. (2002) Trends. Biochem. Sci. 27:552.
- Schiavo, G. et al. (2000) Physiol. Rev. 80:717.
Long Name
Botulinum Neurotoxin Type A Light Chain
Alternate Names
BoNTA Light Chain
Entrez Gene IDs
5185061 (C. botulinum)
Gene Symbol
BONT
UniProt
Additional BoNT-A Light Chain Products
Product Documents for Recombinant Botulinum Neurotoxin Type A Light Chain, CF
Product Specific Notices for Recombinant Botulinum Neurotoxin Type A Light Chain, CF
For research use only
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