Recombinant Chicken Caronte Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1051-CA
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity, Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived chicken Caronte protein
| Chicken Caronte (Asp16 - Asn272) and (Glu18 - Asn272) Accession # NP_990154 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp16 & Glu18
Predicted Molecular Mass
56 kDa (monomer)
SDS-PAGE
60-70 kDa, reducing conditions
Activity
Measured by its ability to cause heart reversals when injected into the right lateral plate of a population of Xenopus neurulae embryos.
80 ng per embryo induces 25-50% heart reversals.
Measured by its binding ability in a functional ELISA.
Immobilized rchCaronte/Fc Chimera at 5 µg/mL (100 µL/well) can bind hBMP-4 with a linear range of 0.8-100 ng/mL.
80 ng per embryo induces 25-50% heart reversals.
Measured by its binding ability in a functional ELISA.
Immobilized rchCaronte/Fc Chimera at 5 µg/mL (100 µL/well) can bind hBMP-4 with a linear range of 0.8-100 ng/mL.
Reviewed Applications
Read 1 review rated 5 using 1051-CA in the following applications:
Formulation, Preparation and Storage
1051-CA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Caronte
Chicken Caronte, the ortholog of mammalian Cerberus, is a member of the Cerberus/DAN family of secreted glycoproteins. There are at least 5 Dan Domain (DAND) family members including DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), Cerberus (DAND4), and Coco (DAND5). Caronte is expressed in the left paraxial mesoderm and is the molecule responsible for transducing left-sided positional information from the node to the periphery of the lateral plate mesoderm (LPM) during chicken embryonic development. Caronte functions as a BMP antagonist, inducing Nodal and downstream genes in the LPM by interfering with the repressive activity of bilaterally-produced BMPs. In addition, antagonism of BMPs by Caronte induces the expression of the Lefty gene in the midline. Caronte binds both BMP-4 and BMP-7 in immunoprecipitation experiments. Recombinant chicken Caronte preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA and induce heart reversals in Xenopus embryos.
References
- Pearce, J. et al. (1999) Dev. Biol. 209:98.
- Yokouchi, Y. et al. (1999) Cell. 98:573.
- King, T. and N. Brown (1999) Nature 401:222.
- Ahu, L. et al. (1999) Curr. Biol. 9:931.
- Rodriguez-Esteban, C. et al. (1999) Nature 401:243.
Alternate Names
Chicken Caronte
Entrez Gene IDs
395623 (Chicken)
Gene Symbol
CER1
UniProt
Additional Caronte Products
Product Documents for Recombinant Chicken Caronte Fc Chimera Protein, CF
Product Specific Notices for Recombinant Chicken Caronte Fc Chimera Protein, CF
For research use only
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