Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10027-AL

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

XP_005548303

Conjugate

Unconjugated

Applications

Bioactivity

View all ALCAM/CD166 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey ALCAM/CD166 protein
Trp28-Ala526, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Trp28

Predicted Molecular Mass

57 kDa

SDS-PAGE

67-89 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Cynomolgus Monkey ALCAM/CD166 is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CD6 Fc Chimera binds with an ED50 of 10-50 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

Recombinant Cynomolgus Monkey ALCAM His-tag Protein Binding Activity

Recombinant Cynomolgus Monkey ALCAM His-tag Protein Binding Activity

When Recombinant Cynomolgus Monkey ALCAM (Catalog # 10027-AL) is coated onto a microplate at 1 µg/mL, Biotinylated Recombinant Human CD6 Fc Chimera binds with an ED50 of 10-50 ng/mL.
Recombinant Cynomolgus Monkey ALCAM His-tag Protein SDS-PAGE

Recombinant Cynomolgus Monkey ALCAM His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey ALCAM/CD166 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-89 kDa.

Formulation, Preparation and Storage

10027-AL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: ALCAM/CD166

ALCAM (activated leukocyte cell adhesion molecule), designated CD166, is a 100‑110 kDa type I transmembrane glycoprotein and a member of the Ig CAM family within the immunoglobulin superfamily (1). The cynomologous ALCAM amino acid (aa) sequence includes a signal peptide, an extracellular domain (ECD) with two V‑type and three C2‑type Ig‑like domains, a transmembrane domain and a short cytoplasmic domain (1). Human ALCAM has several isoforms, including an isoform lacking most of the cytoplasmic domain and a secreted isoform (sALCAM) which antagonizes full‑length ALCAM (2, 3). Mature cynomologous ALCAM ECD shares 93% and 96% aa sequence identity with human and mouse/rat ALCAM, respectively. ALCAM is expressed on multiple cell types including thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and monocyte‑derived dendritic cells (1, 4). ALCAM mediates low‑affinity adhesion with itself or the cysteine‑rich scavenger receptor CD6 to regulate T cell development, immunological synapses (IS), and cell migration through endothelial junctions (1‑11). ALCAM on thymic epithelia mediates adhesion to CD6 on CD4+CD8+ T cells (6). Adhesion of ALCAM‑expressing antigen presenting cells and CD6‑expressing T cells stabilizes the early IS, while later it enhances CD3 effects on T cell proliferation, CD25 expression, and Th1 commitment (4, 7, 8). High ALCAM expression at the blood‑brain barrier in active multiple sclerosis, and its mouse model (EAE), promotes leukocyte migration to the brain (8,  9). High ALCAM expression on melanoma cell lines appears to be pro‑metastatic, but anti‑metastatic activity has been reported in breast cancer (3, 10, 11). ALCAM may influence expression or adhesion of the neuronal adhesion molecule NCAM‑L1, both in the developing retina and invasive melanoma (2, 12).

References

  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. van Kilsdonk, J.W.J. et al. (2008) Cancer Res. 68:3671.
  3. Ikeda, K. and T. Quertermous (2004) J. Biol. Chem. 279:55315.
  4. Zimmerman, A.W. et al. (2006) Blood 107:3212.
  5. van Kempen, L.C. et al. (2001) J. Biol. Chem. 276:25783.
  6. Castro, M.A.A. et al. (2007) J. Immunol. 178:4351.
  7. Nair, P. et al. (2010) Clin. Exp. Immunol. 162:116.
  8. Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
  9. Cayrol, R. et al. (2008) Nat. Immunol. 9:137.
  10. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  11. King, J.A. et al. (2010) Mol. Cancer 9:266.
  12. Buhusi, M. et al. (2009) J. Neurosci. 29:15630.

Long Name

Activated Leukocyte Cell Adhesion Molecule

Alternate Names

CD166

Entrez Gene IDs

214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)

Gene Symbol

ALCAM

UniProt

XP_005548303

Additional ALCAM/CD166 Products

Product Documents for Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Cynomolgus Monkey ALCAM His-tag Protein, CF

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